THE INFLUENCE OF DEPOLYMERIZATION OR DISINTEGRATION OF MICROTUBULE SYSTEM OF THE CYTOSKELETON OF NONTRANSFORMED AND TRANSFORMED-CELLS

How important are the changes of microtubule control for the realization of actin cortex changes during neoplastic transformation? To answer this question we studied the actin cytoskeleton and intermediate filaments condition after colcemid distruction or taxol desintegration of microtubule system in non-transformed cells BALB/c 3T3 and in the same cells transformed by Ha-ras gene. We have come to a conclusion that the differences between non-transformed and transformed cells in the actin cytoskeleton organization remain the same after specific inhibitor action on the microtubules; after the microtubules are destroyed the differences between the two cell types appear in the Intermediate filament organization; there are reasons to assume that changes in the actin cortex structure may play the central role in morphological transformation expression.