DORSAL VENTRAL PATTERNING IN DROSOPHILA - DNA-BINDING OF SNAIL PROTEIN TO THE SINGLE-MINDED GENE

被引：91

作者：

KASAI, Y ^{[1
]}

NAMBU, JR ^{[1
]}

LIEBERMAN, PM ^{[1
]}

CREWS, ST ^{[1
]}

机构：

[1] UNIV CALIF LOS ANGELES,INST MOLEC BIOL,DEPT BIOL,LOS ANGELES,CA 90024

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 08期

关键词：

TRANSCRIPTIONAL CONTROL; ZINC FINGERS; CENTRAL NERVOUS SYSTEM DEVELOPMENT;

D O I：

10.1073/pnas.89.8.3414

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The Drosophila snail gene is required for proper mesodermal development. Genetic studies suggest that it functions by repressing adjacent ectodermal gene expression including that of the single-minded (sim) gene. The snail gene encodes a protein with a zinc-finger motif, and here we report that the snail gene product is a sequence-specific DNA binding protein. The snail protein recognizes a 14-base-pair consensus sequence that is found nine times in a 2.8-kilobase sim regulatory region. These results provide evidence for the direct control of sim transcription by snail.

引用

页码：3414 / 3418

页数：5

共 29 条

[21] THE GRADED DISTRIBUTION OF THE DORSAL MORPHOGEN IS INITIATED BY SELECTIVE NUCLEAR TRANSPORT IN DROSOPHILA [J].

RUSHLOW, CA ;

HAN, KY ;

MANLEY, JL ;

LEVINE, M .

CELL, 1989, 59 (06) :1165-1177

[22]

SARGENT MG, 1990, DEVELOPMENT, V109, P967

[23] SEQUENCE-SPECIFIC DNA-BINDING ACTIVITIES OF THE GAP PROTEINS ENCODED BY HUNCHBACK AND KRUPPEL IN DROSOPHILA [J].

STANOJEVIC, D ;

HOEY, T ;

LEVINE, M .

NATURE, 1989, 341 (6240) :331-335

[24] RELOCALIZATION OF THE DORSAL PROTEIN FROM THE CYTOPLASM TO THE NUCLEUS CORRELATES WITH ITS FUNCTION [J].

STEWARD, R .

CELL, 1989, 59 (06) :1179-1188

[25]

STUDIER FW, 1990, METHOD ENZYMOL, V185, P60

[26] A NON-RADIOACTIVE INSITU HYBRIDIZATION METHOD FOR THE LOCALIZATION OF SPECIFIC RNAS IN DROSOPHILA EMBRYOS REVEALS TRANSLATIONAL CONTROL OF THE SEGMENTATION GENE HUNCHBACK [J].

TAUTZ, D ;

PFEIFLE, C .

CHROMOSOMA, 1989, 98 (02) :81-85

[27]

TAYLOR JW, 1985, NUCLEIC ACIDS RES, V13, P8765

[28] SEQUENCE OF THE TWIST GENE AND NUCLEAR-LOCALIZATION OF ITS PROTEIN IN ENDOMESODERMAL CELLS OF EARLY DROSOPHILA EMBRYOS [J].

THISSE, B ;

STOETZEL, C ;

GOROSTIZATHISSE, C ;

PERRINSCHMITT, F .

EMBO JOURNAL, 1988, 7 (07) :2175-2183

[29] MOLECULAR-GENETICS OF THE SINGLE-MINDED LOCUS - A GENE INVOLVED IN THE DEVELOPMENT OF THE DROSOPHILA NERVOUS-SYSTEM [J].

THOMAS, JB ;

CREWS, ST ;

GOODMAN, CS .

CELL, 1988, 52 (01) :133-141

← 1 2 3 →