NEW HIV PLAQUE TITRATION - APPLICATION TO THE ASSAY OF NEUTRALIZING ANTIBODY

被引：1

作者：

TSUNETSUGUYOKOTA, Y

OHSHIMA, M

NAITO, A

CHERMANN, JC

SHIH, J

YOSHIKURA, H

机构：

[1] UNIV TOKYO,FAC MED,DEPT BACTERIOL,BUNKYO KU,TOKYO 106,JAPAN

[2] INSERM,U322,UNITE RECH RETROVIRUS & MALAD ASSOCIEES,F-13258 MARSEILLE 09,FRANCE

[3] NIH,CTR CLIN,DEPT TRANSFUS MED,BETHESDA,MD 20892

来源：

JOURNAL OF VIROLOGICAL METHODS | 1993年 / 41卷 / 01期

关键词：

HUMAN IMMUNODEFICIENCY VIRUS; PLAQUE ASSAY; NEUTRALIZING ANTIBODY;

D O I：

10.1016/0166-0934(93)90162-K

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple, sensitive and accurate plaque assay was developed using HPB-Ma, a variant of the human T-cell line HPB-ALL, which becomes adherent to the substratum after infection with an amphotropic murine sarcoma virus (MSVa). The simplicity of this novel plaque assay allowed us to. examine a large number of serum samples from patients with HIV infection for neutralizing antibody activity against two human immunodeficiency virus type-1 (HIV-1) strains. During the progression of clinical disease, the neutralizing activity in the sera from two individual patients remained unchanged or increased. A patient with a known time of HIV infection produced cross-neutralizing antibody at 25-34 weeks. The neutralizing activity in the sera from 17 asymptomatic carriers, four patients with AIDS-related complex and four AIDS patients was also examined and was found to be unrelated to the clinical stage.

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页码：47 / 57

页数：11

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