LARGE-SCALE GENOMIC SEQUENCING - OPTIMIZATION OF GENOMIC CHEMICAL SEQUENCING REACTIONS

被引：0
作者：
DOLAN, M
ALLY, A
PURZYCKI, MS
GILBERT, W
GILLEVET, PM
机构：
[1] LIFE TECHNOL,ROCKVILLE,MD
[2] HARVARD UNIV,CAMBRIDGE,MA 02138
[3] NIH,CAMBRIDGE,MD
来源：
BIOTECHNIQUES | 1995年 / 19卷 / 02期
关键词：
D O I：
暂无
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We have developed a streamlined reproducible method for performing genomic chemical sequencing reactions on the genomic DNA of Mycoplasma capricolum, which has a genome size of about 750000 base pairs and whose composition is 75% AT The general modifications that ensure reproducibility and allow the processing of multiple samples can be widely adopted to other large-scale sequencing projects, while the specific modifications to the chemical reactions are applicable to the sequencing of other DNAs with a high AT content.
引用
收藏
页码：264 / &
相关论文
共 50 条
  • [41] Large-scale EST sequencing in rice
    Kimiko Yamamoto
    Takuji Sasaki
    Plant Molecular Biology, 1997, 35 : 135 - 144
  • [42] THE ROLE OF ANTICIPATED AND ANTICIPATORY AFFECT IN REACTIONS TO GENOMIC SEQUENCING FEEDBACK
    Gillman, Arielle S.
    Iles, Irina
    Klein, William
    Biesecker, Barbara B.
    Biesecker, Leslie
    Lewis, Katie
    Ferrer, Rebecca
    ANNALS OF BEHAVIORAL MEDICINE, 2020, 54 : S730 - S730
  • [43] Large-Scale Genomic Biobanks and Cardiovascular Disease
    Aeron M. Small
    Christopher J. O’Donnell
    Scott M. Damrauer
    Current Cardiology Reports, 2018, 20
  • [44] Genomic DNA sequencing methods
    Favello, A
    Hillier, L
    Wilson, RK
    METHODS IN CELL BIOLOGY, VOL 48, 1995, 48 : 551 - 569
  • [45] Genomic sequencing in clinical trials
    Mestan, Karen K.
    Ilkhanoff, Leonard
    Mouli, Samdeep
    Lin, Simon
    JOURNAL OF TRANSLATIONAL MEDICINE, 2011, 9
  • [46] Large-Scale Genomic Biobanks and Cardiovascular Disease
    Small, Aeron M.
    O'Donnell, Christopher J.
    Damrauer, Scott M.
    CURRENT CARDIOLOGY REPORTS, 2018, 20 (04)
  • [47] Accelerating Large-Scale Genomic Analysis with Spark
    Li, Xueqi
    Tan, Guangming
    Zhang, Chunming
    Li, Xu
    Zhang, Zhonghai
    Sun, Ninghui
    2016 IEEE INTERNATIONAL CONFERENCE ON BIOINFORMATICS AND BIOMEDICINE (BIBM), 2016, : 747 - 751
  • [48] Sequencing Disparity in the Genomic Era
    David, Kyle T.
    Wilson, Alan E.
    Halanych, Kenneth M.
    MOLECULAR BIOLOGY AND EVOLUTION, 2019, 36 (08) : 1624 - 1627
  • [49] Large-scale genomic analysis of ovarian carcinomas
    Gorringe, Kylie L.
    Campbell, Ian G.
    MOLECULAR ONCOLOGY, 2009, 3 (02): : 157 - 164
  • [50] GENOMIC AMPLIFICATION WITH TRANSCRIPT SEQUENCING
    STOFLET, ES
    KOEBERL, DD
    SARKAR, G
    SOMMER, SS
    SCIENCE, 1988, 239 (4839) : 491 - 494
12345