PRODUCTION OF BOVINE INSULIN-LIKE GROWTH FACTOR-II (BIGF2) IN ESCHERICHIA-COLI

被引：6

作者：

EASTON, AM ^{[1
]}

GIERSE, JK ^{[1
]}

SEETHARAM, R ^{[1
]}

KLEIN, BK ^{[1
]}

KOTTS, CE ^{[1
]}

机构：

[1] MONSANTO CO,DIV ANIM SCI,CHESTERFIELD,MO 63198

来源：

GENE | 1991年 / 101卷 / 02期

关键词：

RECOMBINANT DNA; GENE EXPRESSION; CODON CHOICE; RPOH358; MUTANT; INCLUSION BODIES; SULFITOLYSIS;

D O I：

10.1016/0378-1119(91)90426-C

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Bovine insulin-like growth factor 2 (bIGF2) was produced in inclusion bodies in the cytoplasm of Escherichia coli and accumulated at high levels: 20-25% of total Coomassie-stained bacterial protein. The level of accumulation of bIGF2 was affected by the choice of codons in the 5' end of the coding sequence and by a rpoH mutation in the host cells. Purified recombinant bIGF2 had the native N terminus and the same mitogenic activity as that of bIGF2 purified from bovine serum.

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页码：291 / 295

页数：5

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