A MONOCLONAL-ANTIBODY DISTINGUISHES 2 TYPES OF PHOSPHATIDYLINOSITOL 4-KINASE

被引：45
作者：
ENDEMANN, GC
GRAZIANI, A
CANTLEY, LC
机构：
[1] STANFORD UNIV,MED CTR,SCH MED,DEPT CELL BIOL,STANFORD,CA 94305
[2] TUFTS UNIV,SCH MED,DEPT PHYSIOL,BOSTON,MA 02111
来源：
BIOCHEMICAL JOURNAL | 1991年 / 273卷
关键词：
D O I：
10.1042/bj2730063
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A monoclonal antibody has been developed against the type II PtdIns 4-kinase from bovine brain. This antibody, 4C5G, causes greater than 90 % inhibition of the type II PtdIns 4-kinase from bovine brain, rat brain and human erythrocytes. However, it fails to inhibit type III PtdIns 4-kinase from bovine brain or PtdIns 3-kinase from rat liver. These results suggest that type II and type III PtdIns 4-kinases are distinct gene products, and that 4C5G will be useful in studying the function of the type II PtdIns 4-kinase.
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页码：63 / 66
页数：4
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