MODELING APPROACH TO CONTROL OF CARBOHYDRATE-METABOLISM DURING CITRIC-ACID ACCUMULATION BY ASPERGILLUS-NIGER .2. SENSITIVITY ANALYSIS

被引：51

作者：

TORRES, NV

机构：

[1] Departamento de Bioquímica Y Biología Molecular, Facultad de Biología, Universidad de la Laguna, La Laguna, Tenerife

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1994年 / 44卷 / 01期

关键词：

SENSITIVITY ANALYSIS; CARBOHYDRATE METABOLISM; ASPERGILLUS NIGER; CITRIC ACID; OPTIMIZATION;

D O I：

10.1002/bit.260440116

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Steady state sensitivity analysis of a model of carbohydrate metabolism and anaplerotic synthesis of oxalacetate were, in Aspergillus niger under conditions of citric acid accumulation, carried out. The flux and metabolite concentration control structure of the system obtained shows that the hexokinase/substrate transport step is the main controlling step of the pathway. The quantitative contribution of the other enzyme catalyzed or transport steps are also discussed. These results allow the design of a proper strategy of biotechnological manipulation aimed at improvement of the process. (C) 1994 John Wiley and Sons, Inc.

引用

页码：112 / 118

页数：7

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