DNA IMAGE-ANALYSIS IN CHILDHOOD ACUTE LYMPHOBLASTIC-LEUKEMIA

被引：9

作者：

CZADER, M ^{[1
]}

PORWIT, A ^{[1
]}

SODERHALL, S ^{[1
]}

BLENNOW, E ^{[1
]}

WIDELL, S ^{[1
]}

REIZENSTEIN, P ^{[1
]}

OST, A ^{[1
]}

AUER, G ^{[1
]}

机构：

[1] KAROLINSKA HOSP,DEPT PATHOL,S-10401 STOCKHOLM 60,SWEDEN

来源：

LEUKEMIA & LYMPHOMA | 1993年 / 9卷 / 03期

关键词：

CHILDHOOD ALL; IMAGE CYTOMETRY; DNA ANEUPLOIDY;

D O I：

10.3109/10428199309147375

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

DNA index (DI) and percentages of cells in S and G2/M phase were determined in Feulgen stained nuclei of blasts from 31 cases of childhood ALL at diagnosis. In 6 cases the results of DNA analysis and cytogenetics were concordant showing hyperdiploidy. Two other cases with normal karyotype were revealed as DNA aneuploid with image analysis. Cases with cytogenetic abnormalities like translocation, deletion or presence of single or double supernumerary chromosomes had DI within normal ranges. Nine ALL cases (29%) were found to be DNA aneuploid-8 hyperdiploid and 1 hypodiploid. The percentages of cells in S and G2/M phase for blasts from bone marrow (mean 17.6%) were significantly higher than those estimated in the peripheral blood (mean 1.57%). We conclude that analysis by image cytometry can detect aneuploid DNA content even in cases, which showed a normal karyotype and provides new information concerning the biological aspects of leukemic blasts.

引用

页码：229 / 235

页数：7

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