DEVELOPMENT AND APPLICATION OF A MICRONEUTRALIZATION ELISA FOR THE DETECTION OF ANTIBODIES TO BOVINE RESPIRATORY SYNCYTIAL VIRUSES

被引：5

作者：

ELLIS, JA ^{[1
]}

HASSARD, L ^{[1
]}

MORLEY, PS ^{[1
]}

机构：

[1] UNIV SASKATCHEWAN,WESTERN COLL VET MED,DEPT VET INTERNAL MED,SASKATOON,SK S7N 0W0,CANADA

来源：

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION | 1995年 / 7卷 / 02期

关键词：

D O I：

10.1177/104063879500700203

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

A microneutralization enzyme-linked immunosorbent assay (ELISA) was developed to detect specific antibodies to bovine respiratory syncytial viruses (BRSVs) in cattle sera using a monoclonal antibody to the fusion protein of the virus. Serum from 20 naturally exposed, 24 experimentally infected, and 15 immunized cattle were evaluated using 3 different BRSV isolates. Antibody titers determined with the microneutralization ELISA were compared with those derived from a classical virus neutralization assay, an indirect ELISA, and a fusion inhibition assay. These studies demonstrated a high degree of correlation (usually 0.90) among the assays. Furthermore, the results showed that immunization of cattle with one isolate (subgroup) of BRSV induced antibody responses that cross-reacted with at least 2 disparate isolates. These results document the utility of the microneutralization ELISA in assessing functionally important antibody responses to BRSVs in cattle.

引用

页码：183 / 189

页数：7

共 22 条

[1]

Anderson L.J., Hendry R.M., Pierik L.T., Multicenter study of strains of respiratory syncytial virus, J Infect Dis, 163, pp. 626-633, (1991)

[2]

Anderson L.J., Hierholzer J.C., Bingham P.G., Stone Y.O., Microneutralization test for respiratory syncytial virus based on an enzyme immunoassay, J Clin Microbiol, 22, pp. 1050-1052, (1985)

[3]

Baker J.C., Frey M.L., Bovine respiratory syncytial virus, Vet Clin North Am (Food Anim Pract), 1, pp. 259-275, (1985)

[4]

Baker J.C., Werdin R.E., Ames T.R., Et al., Study on the etiologic role of bovine respiratory syncytial virus in pneumonia of dairy calves, J Am Vet Med Assoc, 189, pp. 66-70, (1986)

[5]

Baker J.C., Wilson E.G., McKay G.L., Et al., Identification of subgroups of bovine respiratory syncytial virus, J Clin Microbiol, 30, pp. 1120-1126, (1982)

[6]

Beeler J.A., van Wyke Coelingh K., Neutralization epitopes of the F glycoprotein of respiratory syncytial virus: Effect of mutation upon fusion function, J Virol, 63, pp. 2941-2950, (1989)

[7]

Belknap E.B., Baker J.C., Patterson J.S., Et al., The role of passive immunity in bovine respiratory syncytial virus-infected calves, J Infect Dis, 163, pp. 470-476, (1991)

[8]

Dubovi E.J., Diagnosing B.R.S.V infection: A laboratory perspective, Vet Med (Prague), pp. 888-893, (1993)

[9]

Ellis J.A., Hassard L.E., Morley P.S., Comparative analysis bovine respiratory syncytial virus-specific antibody and T lymphocyte responses in cattle following immunization with commercial polyvalent vaccines, J Am Vet Med Assoc, (1994)

[10]

Ellis J.A., Russell H.I., Cavender J., Haven T., Bovine respiratory syncytial virus-specific immune responses in cattle following immunization with modified-live and inactivated vaccines. Analysis of specificity and activity of serum antibodies, Vet Immunol Immunopathol, 34, pp. 35-45, (1992)

← 1 2 3 →