EXPERIMENTAL AND THEORETICAL DESCRIPTION OF THE ISOTACHOPHORETIC BEHAVIOR OF SERUM-ALBUMIN

被引：19

作者：

THORMANN, W ^{[1
]}

MOSHER, RA ^{[1
]}

机构：

[1] UNIV ARIZONA,CTR SEPARAT SCI,TUCSON,AZ 85721

来源：

ELECTROPHORESIS | 1990年 / 11卷 / 04期

关键词：

D O I：

10.1002/elps.1150110404

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The isotachophoretic behavior of serum albumin is examined for three anionic and one cationic electrolyte systems by (i) computer simulation, (ii) capillary isotachophoresis (ITP) and (iii) continuous flow ITP. The theoretical relationship between pH of the leading electrolyte and the steady state protein plateau concentration is presented for one of the anionic systems. With leading ion concentrations of the order of 10 mm, experimental protein plateau concentrations of 1.3–2.3 % w/v are obtained. The computer predictions are approximately half these values. Copyright © 1990 VCH Verlagsgesellschaft mbH

引用

页码：292 / 298

页数：7

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