SATURATION OF THE PROCESSING OF NEWLY SYNTHESIZED RIBOSOMAL-RNA IN ISOLATED BRAIN MITOCHONDRIA

被引：20

作者：

ENRIQUEZ, JA ^{[1
]}

LOPEZPEREZ, MJ ^{[1
]}

MONTOYA, J ^{[1
]}

机构：

[1] UNIV ZARAGOZA,FAC VET,DEPT BIOQUIM & BIOL MOLEC & CELULAR,MIGUEL SERVET 177,E-50013 ZARAGOZA,SPAIN

来源：

FEBS LETTERS | 1991年 / 280卷 / 01期

关键词：

MITOCHONDRIA; RNA SYNTHESIS; RNA PROCESSING; ISOLATED ORGANELLE; RAT BRAIN;

D O I：

10.1016/0014-5793(91)80197-B

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Isolated rat brain mitochondria, when incubated in the presence of [alpha-P-32]UTP in an appropriate incubation buffer, in which the energy requirements are provided by exogenous ADP in the presence of an oxidizable substrate, are able to support mitochondrial DNA transcription and RNA processing in a way faithfully resembling the in vivo process. Furthermore, we have strikingly found a saturation of the synthesis of mature 16 S and 12 S rRNA under conditions in which their RNA precursor as well as the mature mRNAs continue being synthesized. This suggests that synthesis of mature rRNAs could be regulated at the level of processing of their precursor rather than at the level of transcription.

引用

页码：32 / 36

页数：5

共 20 条

[1] FRACTIONATION OF MITOCHONDRIAL RNA FROM HELA-CELLS BY HIGH-RESOLUTION ELECTROPHORESIS UNDER STRONGLY DENATURING CONDITIONS [J].