THE CANDIDATE PROTOONCOGENE BCL-3 ENCODES A TRANSCRIPTIONAL COACTIVATOR THAT ACTIVATES THROUGH NF-KAPPA-B P50 HOMODIMERS

被引：354
作者：
FUJITA, T [1 ]
NOLAN, GP [1 ]
LIOU, HC [1 ]
SCOTT, ML [1 ]
BALTIMORE, D [1 ]
机构：
[1] ROCKEFELLER UNIV,NEW YORK,NY 10021
来源：
GENES & DEVELOPMENT | 1993年 / 7卷 / 7B期
关键词：
NF-KAPPA-B; P50; I-KAPPA-B; TRANSCRIPTION; COACTIVATOR;
D O I：
10.1101/gad.7.7b.1354
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The candidate proto-oncogene bcl-3 encodes a protein that shares structural features with IkappaB-alpha and other proteins that bind to members of the Rel protein family. Here, we show that in contrast to the inhibitory activity of IkappaB-alpha, the bcl-3 gene product superactivates NF-kappaB p50 homodimer-mediated gene expression both in vivo and in vitro. BCL-3 protein can, as well, selectively associate with p50 homodimers in the presence of DNA containing a kappaB motif. These results strongly suggest that BCL-3 can act as a transcriptional coactivator, acting through DNA-bound p50 homodimers.
引用
收藏
页码：1354 / 1363
页数：10
相关论文
共 45 条
[21]   DIRECT ASSOCIATION OF PP40/I-KAPPA-B-BETA WITH REL NF-KAPPA-B TRANSCRIPTION FACTORS - ROLE OF ANKYRIN REPEATS IN THE INHIBITION OF DNA-BINDING ACTIVITY [J].
INOUE, JI ;
KERR, LD ;
RASHID, D ;
DAVIS, N ;
BOSE, HR ;
VERMA, IM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (10) :4333-4337
[22]   TNF STIMULATES EXPRESSION OF MOUSE MHC CLASS-I GENES BY INDUCING AN NF-CHI-B-LIKE ENHANCER BINDING-ACTIVITY WHICH DISPLACES CONSTITUTIVE FACTORS [J].
ISRAEL, A ;
LEBAIL, O ;
HATAT, D ;
PIETTE, J ;
KIERAN, M ;
LOGEAT, F ;
WALLACH, D ;
FELLOUS, M ;
KOURILSKY, P .
EMBO JOURNAL, 1989, 8 (12) :3793-3800
[23]   A COMMON POSITIVE TRANS-ACTING FACTOR BINDS TO ENHANCER SEQUENCES IN THE PROMOTERS OF MOUSE H-2-MICROGLOBULIN AND BETA2-MICROGLOBULIN GENES [J].
ISRAEL, A ;
KIMURA, A ;
KIERAN, M ;
YANO, O ;
KANELLOPOULOS, J ;
LEBAIL, O ;
KOURILSKY, P .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (09) :2653-2657
[24]   THE PROTOONCOGENE BCL-3 ENCODES AN I-KAPPA-B PROTEIN [J].
KERR, LD ;
DUCKETT, CS ;
WAMSLEY, P ;
ZHANG, Q ;
CHIAO, P ;
NABEL, G ;
MCKEITHAN, TW ;
BAEUERLE, PA ;
VERMA, IM .
GENES & DEVELOPMENT, 1992, 6 (12A) :2352-2363
[25]   TRANSCRIPTIONAL REGULATION OF THE HIV-1 PROMOTER BY NF-KAPPA-B INVITRO [J].
KRETZSCHMAR, M ;
MEISTERERNST, M ;
SCHEIDEREIT, C ;
LI, G ;
ROEDER, RG .
GENES & DEVELOPMENT, 1992, 6 (05) :761-774
[26]   THE NF-KAPPA-B P50 PRECURSOR, P105, CONTAINS AN INTERNAL I-KAPPA-B-LIKE INHIBITOR THAT PREFERENTIALLY INHIBITS P50 [J].
LIOU, HC ;
NOLAN, GP ;
GHOSH, S ;
FUJITA, T ;
BALTIMORE, D .
EMBO JOURNAL, 1992, 11 (08) :3003-3009
[27]   EUKARYOTIC EXPRESSION VECTORS FOR THE ANALYSIS OF MUTANT PROTEINS [J].
MATTHIAS, P ;
MULLER, MM ;
SCHREIBER, E ;
RUSCONI, S ;
SCHAFFNER, W .
NUCLEIC ACIDS RESEARCH, 1989, 17 (15) :6418-6418
[28]   A QUANTITATIVE-ANALYSIS OF NUCLEAR FACTOR-I DNA INTERACTIONS [J].
MEISTERERNST, M ;
GANDER, I ;
ROGGE, L ;
WINNACKER, EL .
NUCLEIC ACIDS RESEARCH, 1988, 16 (10) :4419-4435
[29]   THE PROLINE-RICH TRANSCRIPTIONAL ACTIVATOR OF CTF/NF-I IS DISTINCT FROM THE REPLICATION AND DNA-BINDING DOMAIN [J].
MERMOD, N ;
ONEILL, EA ;
KELLY, TJ ;
TJIAN, R .
CELL, 1989, 58 (04) :741-753
[30]   THE C-TERMINUS OF THE NF-KAPPA-B P50 PRECURSOR AND AN I-KAPPA-B ISOFORM CONTAIN TRANSCRIPTION ACTIVATION DOMAINS [J].
MORIN, PJ ;
GILMORE, TD .
NUCLEIC ACIDS RESEARCH, 1992, 20 (10) :2453-2458
12345