DECREASED COOMASSIE BRILLIANT BLUE COLOR YIELD WITH GLYCATED PROTEINS

被引:4
作者
SYROVY, I
机构
[1] Institute of Physiology, Czechoslovak Academy of Sciences, Prague
来源
JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1992年 / 25卷 / 01期
关键词
GLYCATED PROTEIN; COOMASSIE BRILLIANT BLUE G-250 COLOR YIELD; PROTEIN DETERMINATION;
D O I
10.1016/0165-022X(92)90038-C
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Collagen, myosin and albumin were incubated for 7 days at 20-degrees-C with fructose, ribose or glyceraldehyde. For thus-formed glycated proteins, quantities were determined by the Conway microdiffusion technique and by the colorimetric method based on Coomassie brilliant blue G-250 colour yield. It was found that when albumin was glycated with increasing amounts of glyceraldehyde, the colour yield was decreased by 7-33%. In collagen, myosin and albumin incubated with 0.5 mol/l fructose, 0.5 mol/l ribose or 0.1 mol/l glyceraldehyde, protein concentration was not changed, as proved by the Conway microdiffusion technique; the Coomassie brilliant blue G-250 colour yield was up to 50% lower, depending on the protein used, and was decreased much less when proteins were incubated with less sugar.
引用
收藏
页码:75 / 78
页数:4
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