SINGLE-CRYSTALS OF V-AMYLOSE COMPLEXED WITH ISOPROPANOL AND ACETONE

Single crystals of amylose complexed with isopropanol or acetone were prepared by adding these precipatants to a metastable aqueous solution of amylose. With both precipitants, similar micrometre sized platelet crystals were obtained. They gave indistinguishable electron diffraction diagrams which could be indexed in an orthorhombic unit cell, with a = 28.26 A ̊, b = 29.30 A ̊, c = 8.01 A ̊ and in a space group P212121 or P21212. Within the unit cell, the amylose chains are organized in antiparallel pairs of parallel 65 amylose helices occupying 70% of the cell content, the remaining 30% consisting of isopropanol/acetone and water, with an estimate of 10 isopropanol/acetone molecules for 52 water molecules per unit cell. If the crystals are suspended in pure isopropanol at various temperatures or in pure methanol at room temperature, they undergo a de-solvation process that ultimately converts them into VH amylose. De-solvation with isopropanol left the crystals intact whereas with methanol, they became cracked during the shrinkage. An explanation is proposed for such difference. © 1990.