STRUCTURAL-PROPERTIES OF THE OUTER-MEMBRANE AND THE REGULAR SURFACE PROTEIN OF COMAMONAS-ACIDOVORANS

被引：34

作者：

ENGELHARDT, H ^{[1
]}

GERBLRIEGER, S ^{[1
]}

KREZMAR, D ^{[1
]}

SCHNEIDERVOSS, S ^{[1
]}

ENGEL, A ^{[1
]}

BAUMEISTER, W ^{[1
]}

机构：

[1] UNIV BASEL,BIOCTR,MAURICE E MULLER INST HIGH RESOLUT ELECTRON MICROSCOPY,CH-4056 BASEL,SWITZERLAND

来源：

JOURNAL OF STRUCTURAL BIOLOGY | 1990年 / 105卷 / 1-3期

关键词：

D O I：

10.1016/1047-8477(90)90103-J

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The outer membrane of Comamonas acidovorans, formerly Pseudomonas acidovorans, contains a regularly arrayed surface protein. The tetragonal lattice (p4 symmetry, unit cell dimensions a = b = 10.5 nm is composed of a single type of polypeptide. It forms dimeric morphological complexes as revealed by means of electron microscopy in conjunction with image processing, STEM mass determination, and IR analysis. The surface protein has tightly associated carbohydrates and behaves like a glycoprotein in electrophoresis and IR spectroscopy. The outer membrane proteins Omp21 and Omp32 are not regularly arrayed. Omp32 has the characteristic attributes of an intrinsic outer membrane protein, such as moderate hydrophobicity, a high β-structure content, and a typical solubilization behavior. It forms channels in black lipid membranes and it, therefore, represents the major porin of C. acidovorans. © 1990.

引用

页码：92 / 102

页数：11

共 54 条

[1] ELECTRON-MICROSCOPY OF AGING CELLS OF PSEUDOMONAS-RHODOS - FINE-STRUCTURE OF NATIVE AND ISOLATED TUBULAR MEMBRANES [J].