EFFECT OF PLATELET-RICH PLASMA MIXED WITH A POLYMERIC BONE FILLING MATERIAL ON SINUS FLOOR AUGMENTATION IN RABBITS

Sinus augmentation is a common approach for patients with severe alveolar ridge atrophy but an ideal material to increase the bone volume for dental implantation is still needed. The present study evaluated the effect of a newly developed polymeric bone-flling powder ( formed from acrylic acid and N-isopropylacrylamide, ANa) mixed with platelet-rich plasma (PRP) for bone generation in a rabbit model of sinus augmentation. A total of 12 New Zealand White rabbits were randomly divided into three groups based on filling material. All animals underwent a bilateral maxillary sinus augmentation. PRP was prepared using an automatic separation system to obtain a high platelet concentration. ANa powder was individually mixed with phosphate-buffered saline (PBS) or PRP for sinus floor elevation. The left maxillary sinus received the ANa/PBS filling, whereas the right maxillary sinus received a mixture of ANa/PRP gel. Equal volumes of filling material were inserted in each maxillary sinus. Thus, defects with no implantation served as controls. Animals were sacrificed at 4 and 12 weeks, and then all specimens were harvested for micro-computed tomography (micro-CT) and histological analysis. On micro-CT evaluation, ANa/PRP significantly increased the bone volume in maxillary sinus augmentation relative to the negative control and ANa/PBS after 12 weeks. New bone areas with osteocytes and osteon formation were found in all three groups at 12 weeks post-implantation. This study confirms that ANa mixed with PRP can effectively increase the bone volume in the sinuscavity. This benefit for sinus augmentation may increase the success of dental implantation because of the formation of a thin layer of alveolar bone.