TEMPORARY LOSS OF GLUCOCORTICOID RECEPTOR-MEDIATED REGULATION OF GENE-EXPRESSION IN HEAT-SHOCKED CELLS

被引：10

作者：

MITSIOU, DJ ^{[1
]}

ALEXIS, MN ^{[1
]}

机构：

[1] NATL HELLEN RES FDN, INST BIOL RES & BIOTECHNOL, GR-11635 ATHENS, GREECE

来源：

FEBS LETTERS | 1995年 / 362卷 / 03期

关键词：

GLUCOCORTICOID RECEPTOR; HEAT SHOCK; GENE EXPRESSION;

D O I：

10.1016/0014-5793(95)00263-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect of heat shock on the transcriptional activity of glucocorticoid receptor was assessed using HeLa cells stably transfected with the chloramphenicol acetyltransferase (CAT) gene the transcription of which is controlled by two glucocorticoid-responsive elements placed directly upstream of a core promoter. Heat shock inactivated the high-affinity glucocorticoid binding capacity of the cells and nullified the rate of accumulation of CAT mRNA in the presence of hormone. Hormonal responsiveness was restored on return to normal temperature concomitantly with recovery of high-affinity glucocorticoid binding capacity. Heat inactivation of the receptor was coincident with loss of its solubility and apparently unrelated to receptor degradation.

引用

页码：309 / 315

页数：7

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