EPIDERMAL GROWTH-FACTOR INDUCES THE DIFFERENTIAL RELEASE OF GP2 AND AMYLASE FROM AR4-2J CELLS

被引：2

作者：

KOSHLUKOVA, S ^{[1
]}

RUBIN, RP ^{[1
]}

WITHIAMLEITCH, M ^{[1
]}

ALETTA, JM ^{[1
]}

机构：

[1] SUNY BUFFALO,SCH MED & BIOMED SCI,DEPT PHARMACOL & TOXICOL,BUFFALO,NY 14214

来源：

CELLULAR SIGNALLING | 1995年 / 7卷 / 06期

关键词：

EPIDERMAL GROWTH FACTOR; GP2; AR4-2J PANCREATOMA CELLS; AMYLASE SECRETION; REGULATED SECRETION; CONSTITUTIVE SECRETION;

D O I：

10.1016/0898-6568(95)00025-K

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Epidermal growth factor (EGF) stimulates secretion of glycoprotein 2 (GP2) in a time- and concentration-dependent manner from the AR4-2J pancreatoma cell line. Cell differentiation induced by dexamethasone treatment for 3 d, however, did not significantly alter either basal or EGF-stimulated GP2 release. Basal and EGF-stimulated GP2 release were similarly unaffected by caerulein, which promotes amylase secretion by a regulated route. A brief exposure to cycloheximide profoundly blocked EGF-evoked GP2 secretion. Furthermore, EGF-stimulated GP2 release was not accompanied by significant alterations in intracellular ionic calcium levels, in contrast to the stimulatory actions of caerulein. We conclude that EGF-stimulated release of GP2 occurs via a novel secretory pathway that is neither regulated nor constitutive as currently defined.

引用

页码：559 / 569

页数：11