CLONING OF A CREATINASE GENE FROM PSEUDOMONAS-PUTIDA IN ESCHERICHIA-COLI BY USING AN INDICATOR PLATE

被引：12
作者：
CHANG, MC
CHANG, CC
CHANG, JC
机构：
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1992年 / 58卷 / 10期
关键词：
D O I：
10.1128/AEM.58.10.3437-3440.1992
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A genomic library of Pseudomonas putida DNA was constructed by using plasmid pBR322. Transformants of Escherichia coli in combination with Proteus mirabilis cells grown on creatinase test plates were screened for creatinase activity; transformants were considered positive for creatinase activity if a red-pink zone appeared around the colonies. One creatinase-positive clone was further analyzed, and the gene was reduced to a 2.7-kb DNA fragment. A unique protein band (with a molecular weight of approximately 50,000) was observed in recombinant E. coli by minicell analysis.
引用
收藏
页码：3437 / 3440
页数：4
相关论文
共 50 条
[41]   A FAMILY OF POSITIVE REGULATORS RELATED TO THE PSEUDOMONAS-PUTIDA TOL PLASMID XYLS AND THE ESCHERICHIA-COLI ARAC ACTIVATORS [J].
RAMOS, JL ;
ROJO, F ;
ZHOU, L ;
TIMMIS, KN .
NUCLEIC ACIDS RESEARCH, 1990, 18 (08) :2149-2152
[42]   TRANSFORMATION OF PSEUDOMONAS-PUTIDA AND ESCHERICHIA-COLI WITH PLASMID-LINKED DRUG-RESISTANCE FACTOR DNA [J].
CHAKRABARTY, AM ;
MYLROIE, JR ;
FRIELLO, DA ;
VACCA, JG .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1975, 72 (09) :3647-3651
[43]   MOLECULAR-CLONING AND SEQUENCING OF THE PHENOL HYDROXYLASE GENE FROM PSEUDOMONAS-PUTIDA BH [J].
TAKEO, M ;
MAEDA, Y ;
OKADA, H ;
MIYAMA, K ;
MORI, K ;
IKE, M ;
FUJITA, M .
JOURNAL OF FERMENTATION AND BIOENGINEERING, 1995, 79 (05) :485-488
[44]   CLONING AND SEQUENCE-ANALYSIS OF THE NTRA (RPON) GENE OF PSEUDOMONAS-PUTIDA [J].
INOUYE, S ;
YAMADA, M ;
NAKAZAWA, A ;
NAKAZAWA, T .
GENE, 1989, 85 (01) :145-152
[45]   PSEUDOMONAS PUTIDA GENES RESPONSIBLE FOR PRIMARY STAGES OF NAPHTHALENE OXIDATION - CLONING IN ESCHERICHIA-COLI CELLS [J].
BORONIN, AM ;
TSOI, TV ;
KOSHELEVA, IA ;
ARINBASAROV, MU ;
ADANIN, VM ;
SELIFONOV, SA ;
KOZLOVSKII, AG .
GENETIKA, 1989, 25 (02) :226-237
[46]   CLONING OF EXO-MALTOTETRAOHYDROLASE GENE FROM PSEUDOMONAS-SACCHAROPHILA IN ESCHERICHIA-COLI [J].
ZHOU, J ;
TAKANO, T ;
KOBAYASHI, S .
AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1989, 53 (01) :301-302
[47]   INCREASED EXPRESSION OF THE PLASMID-DETERMINED 2,3-DIHYDROXYBIPHENYL DIOXYGENASE GENE IN STRAINS OF ESCHERICHIA-COLI, PSEUDOMONAS-PUTIDA AND PSEUDOMONAS-AERUGINOSA [J].
ANDREYEVA, AL ;
SLEPENKYN, AV ;
STAROVOYTOV, II .
FEMS MICROBIOLOGY LETTERS, 1993, 106 (02) :211-216
[48]   STEREOSPECIFIC HYDROXYLATION OF INDAN BY ESCHERICHIA-COLI CONTAINING THE CLONED TOLUENE DIOXYGENASE GENES FROM PSEUDOMONAS-PUTIDA F1 [J].
BRAND, JM ;
CRUDEN, DL ;
ZYLSTRA, GJ ;
GIBSON, DT .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1992, 58 (10) :3407-3409
[49]   DETERMINATION OF GOLD BY SLURRY ELECTROTHERMAL ATOMIC-ABSORPTION SPECTROMETRY AFTER PRECONCENTRATION BY ESCHERICHIA-COLI AND PSEUDOMONAS-PUTIDA [J].
ROBLES, LC ;
GARCIAOLALLA, C ;
ALLER, AJ .
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY, 1993, 8 (07) :1015-1022
[50]   KINETICS OF DEOXYRIBONUCLEIC ACID DESTRUCTION AND SYNTHESIS DURING GROWTH OF BDELLOVIBRIO-BACTERIOVORUS ON PSEUDOMONAS-PUTIDA AND ESCHERICHIA-COLI [J].
MATIN, A .
ANTONIE VAN LEEUWENHOEK JOURNAL OF MICROBIOLOGY, 1972, 38 (04) :630-630
12345