TRICYCLIC ANTIDEPRESSANT ANALYSIS BY REVERSED PHASE LIQUID-CHROMATOGRAPHY USING PHENYL COLUMNS

被引：6

作者：

WONG, SHY ^{[1
]}

MCHUGH, SL ^{[1
]}

DOLAN, J ^{[1
]}

COHEN, KA ^{[1
]}

机构：

[1] IBM INSTRUMENTS INC, DANBURY, CT 06810 USA

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1986年 / 9卷 / 11期

关键词：

CHROMATOGRAPHIC ANALYSIS - Column - ORGANIC COMPOUNDS - Chromatographic Analysis;

D O I：

10.1080/01483918608076881

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Tricyclic antidepressant (TCA) analysis by reversed-phase liquid chromatography was achieved by a novel approach using two mini phenyl columns (4. 5 multiplied by 50 mm). Analyses were organized into two drug groups: 1. n-desmethyl doxepin, doxepin, nortriptyline and amitriptyline, and 2. desipramine and imipramine. Chromatographic parameters were: two mini phenyl columns; mobile phase 0. 01 M n-nonylamine in 0. 02 M NaH//2PO//4, pH equals 3. 0/ACN (9:1); flow rate 3. 0 ml/min. ; ambient temperature; sample volume 50 ul, and detection wavelength 214 nm at 0. 01 to 0. 05 AUFS. Analysis time was about 8 minutes. Peak height ratios were linearly correlated to concentrations ranging from 25 to 800 mu g/L. Criteria are suggested for using this procedure as a viable alternative for TCAs monitoring.

引用

页码：2511 / 2538

页数：28