TUNGSTEN TREATMENT PREVENTS TUMOR NECROSIS FACTOR-INDUCED INJURY OF BRAIN ENDOTHELIAL-CELLS

被引：23
作者：
TERADA, LS
WILLINGHAM, IR
GUIDOT, DM
SHIBAO, GN
KINDT, GW
REPINE, JE
机构：
[1] UNIV COLORADO,HLTH SCI CTR,DEPT MED,DENVER,CO 80262
[2] UNIV COLORADO,HLTH SCI CTR,DEPT NEUROSURG,DENVER,CO 80262
来源：
INFLAMMATION | 1992年 / 16卷 / 01期
关键词：
D O I：
10.1007/BF00917511
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Exposure to recombinant human tumor necrosis factor-alpha (TNF-alpha) or calcium ionophore (A23187) for 4 h increased (P < 0.05) lactate dehydrogenase (LDH) release from cultured bovine brain endothelial cells (EC). In contrast, treatment with endotoxin or interleukin-1 did not increase (P > 0.05) LDH release from brain EC. Pretreatment with tungsten decreased (P < 0.05) xanthine oxidase activity in brain EC and decreased (P < 0.05) LDH release from brain EC following exposure to TNF. Our results suggest that TNF-alpha injures brain microvascular EC and that this effect may be mediated by xanthine oxidase.
引用
收藏
页码：13 / 19
页数：7
相关论文
共 27 条
[1]   ROLE OF XANTHINE-OXIDASE AND NEUTROPHILS IN ISCHEMIA-REPERFUSION INJURY IN RABBIT LUNG [J].
ADKINS, WK ;
TAYLOR, AE .
JOURNAL OF APPLIED PHYSIOLOGY, 1990, 69 (06) :2012-2018
[2]   HYPEROXIA DAMAGES CULTURED ENDOTHELIAL-CELLS CAUSING INCREASED NEUTROPHIL ADHERENCE [J].
BOWMAN, CM ;
BUTLER, EN ;
REPINE, JE .
AMERICAN REVIEW OF RESPIRATORY DISEASE, 1983, 128 (03) :469-472
[3]   BRAIN MICROVESSEL ENDOTHELIAL-CELLS IN TISSUE-CULTURE - A MODEL FOR STUDY OF BLOOD-BRAIN-BARRIER PERMEABILITY [J].
BOWMAN, PD ;
ENNIS, SR ;
RAREY, KE ;
BETZ, AL ;
GOLDSTEIN, GW .
ANNALS OF NEUROLOGY, 1983, 14 (04) :396-402
[4]   TUMOR NECROSIS FACTOR CACHECTIN INCREASES PERMEABILITY OF ENDOTHELIAL-CELL MONOLAYERS BY A MECHANISM INVOLVING REGULATORY G-PROTEINS [J].
BRETT, J ;
GERLACH, H ;
NAWROTH, P ;
STEINBERG, S ;
GODMAN, G ;
STERN, D .
JOURNAL OF EXPERIMENTAL MEDICINE, 1989, 169 (06) :1977-1991
[5]   ANTIOXIDANTS PROTECT CULTURED BOVINE LUNG ENDOTHELIAL-CELLS FROM INJURY BY ENDOTOXIN [J].
BRIGHAM, KL ;
MEYRICK, B ;
BERRY, LC ;
REPINE, JE .
JOURNAL OF APPLIED PHYSIOLOGY, 1987, 63 (02) :840-850
[6]   INTERLEUKIN-1 PRETREATMENT DECREASES ISCHEMIA REPERFUSION INJURY [J].
BROWN, JM ;
WHITE, CW ;
TERADA, LS ;
GROSSO, MA ;
SHANLEY, PF ;
MULVIN, DW ;
BANERJEE, A ;
WHITMAN, GJR ;
HARKEN, AH ;
REPINE, JE .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (13) :5026-5030
[7]   XANTHINE-OXIDASE PRODUCES HYDROGEN-PEROXIDE WHICH CONTRIBUTES TO REPERFUSION INJURY OF ISCHEMIC, ISOLATED, PERFUSED RAT HEARTS [J].
BROWN, JM ;
TERADA, LS ;
GROSSO, MA ;
WHITMANN, GJ ;
VELASCO, SE ;
PATT, A ;
HARKEN, AH ;
REPINE, JE .
JOURNAL OF CLINICAL INVESTIGATION, 1988, 81 (04) :1297-1301
[8]   MEDIATOR-INDUCED ACTIVATION OF XANTHINE-OXIDASE IN ENDOTHELIAL-CELLS [J].
FRIEDL, HP ;
TILL, GO ;
RYAN, US ;
WARD, PA .
FASEB JOURNAL, 1989, 3 (13) :2512-2518
[9]   TUMOR-NECROSIS-FACTOR (CACHECTIN) AS AN ESSENTIAL MEDIATOR IN MURINE CEREBRAL MALARIA [J].
GRAU, GE ;
FAJARDO, LF ;
PIGUET, PF ;
ALLET, B ;
LAMBERT, PH ;
VASSALLI, P .
SCIENCE, 1987, 237 (4819) :1210-1212
[10]   TUMOR NECROSIS FACTOR IDENTIFIED IN MULTIPLE-SCLEROSIS BRAIN [J].
HOFMAN, FM ;
HINTON, DR ;
JOHNSON, K ;
MERRILL, JE .
JOURNAL OF EXPERIMENTAL MEDICINE, 1989, 170 (02) :607-612
123