CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF (1,3)BETA-D-GLUCANASES AND (1,3,1,4)-BETA-D-GLUCANASES FROM GERMINATING BARLEY

(1,3)-β-D-Glucanase isoenzyme GII and (1,3;1,4)-β-D-glucanase isoenzyme EII from barley have been crystallized by the hanging drop method in the presence of ammonium sulphate. The crystals of the (1,3)-β-D-glucanase, which diffract to about 1.8 Å resolution, belong to the trigonal space group P31212 (or P32212) with cell constants a = b = 86.9 Å, c = 156.0 Å and contain two molecules in the asymmetric unit. The crystals of the (1,3;1,4)-β-D-glucanase which diffract to better than 1.8 Å resolution, belong to the tetragonal space group P43212 (or P41212) with cell constants a = b = 87.4 Å, c = 109.5 Å and contain one molecule in the asymmetric unit.