FLOW-INJECTION ENZYME-IMMUNOASSAY OF HAPTENS WITH ENHANCED CHEMILUMINESCENCE DETECTION

被引：38

作者：

AREFYEV, AA ^{[1
]}

VLASENKO, SB ^{[1
]}

EREMIN, SA ^{[1
]}

OSIPOV, AP ^{[1
]}

EGOROV, AM ^{[1
]}

机构：

[1] MV LOMONOSOV STATE UNIV,DEPT CHEM,DIV CHEM,MOSCOW 117234,USSR

来源：

ANALYTICA CHIMICA ACTA | 1990年 / 237卷 / 02期

关键词：

Enzyme system; Haptens; Thyroxine;

D O I：

10.1016/S0003-2670(00)83930-6

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A non-equilibrium flow-injection enzyme immunoassay for thyroxine (model hapten) involving affinity separation of the immunocomplex of horseradish peroxidase-labelled antibodies and antigen from the free labelled antibodies is described. An enhanced chemiluminescence reaction was used for detection of horseradish peroxidase as an antibody marker. The method allows the determination of thyroxine concentrations as low as 10-11 M within 5 min and the precision is 10% (relative standard deviation). © 1990.

引用

页码：285 / 289

页数：5

共 8 条

[1]

AREFYEV AA, 1987, ZH MIKROBIOL EIDEM I, V9, P27

[2]

OSIPOV AP, 1988, J BIOLUM CHEMILUM, V2, P242

[3]

OSIPOV AP, 1989, ANAL LETT, V22, P1841

[4]

THORPE GHG, 1986, METHOD ENZYMOL, V133, P331

[5]

TIJSSEN P, 1985, LABORATORY TECHNIQUE

[6] AN INVESTIGATION ON THE CATALYTIC MECHANISM OF ENHANCED CHEMI-LUMINESCENCE - IMMUNOCHEMICAL APPLICATIONS OF THIS REACTION [J].

VLASENKO, SB ;

AREFYEV, AA ;

KLIMOV, AD ;

KIM, BB ;

GOROVITS, EL ;

OSIPOV, AP ;

GAVRILOVA, EM ;

YEGOROV, AM .

JOURNAL OF BIOLUMINESCENCE AND CHEMILUMINESCENCE, 1989, 4 (01) :164-176

[7] DETERMINATION OF HUMAN-SERUM IMMUNOGLOBULIN-G USING FLOW-INJECTION ANALYSIS WITH RATE TURBIDIMETRIC DETECTION [J].

WORSFOLD, PJ ;

HUGHES, A ;

MOWTHORPE, DJ .

ANALYST, 1985, 110 (11) :1303-1305

[8] THE BIO-ANALYTICAL POTENTIAL OF FLOW-INJECTION ANALYSIS [J].

WORSFOLD, PJ .

ANALYTICA CHIMICA ACTA, 1983, 145 (JAN) :117-124

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