DETERMINATION OF CATECHOLAMINES IN URINE BY LIQUID-CHROMATOGRAPHY AND ELECTROCHEMICAL DETECTION AFTER ONLINE SAMPLE PURIFICATION ON IMMOBILIZED BORONIC ACID

被引：21
作者：
ERIKSSON, BM
WIKSTROM, M
机构：
来源：
JOURNAL OF CHROMATOGRAPHY | 1992年 / 593卷 / 1-2期
关键词：
D O I：
10.1016/0021-9673(92)80285-3
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Norepinephrine, epinephrine and dopamine in urine were measured by an automated liquid chromatographic method. After sample purification on a column containing silica-immobilized boronic acid, which showed great affinity for catecholamines at neutral pH, the catecholamines were eluted by backflushing with an acidic mobile phase and transferred to a cation exchanger for separation. Detection was performed electrochemically and the relative standard deviation was 2% for the analysis of endogenous concentrations in human urine.
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页码：185 / 190
页数：6
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