Effect of cooling on motility, acrosomal membrane integrity and acrosome reaction in canine spermatozoa

被引：0
|
作者：
Manosalva P, Iris [1 ]
Cortes, Constanza [2 ]
Leyva V, Victor [3 ]
Valdivia C, Martha [1 ]
De los Reyes S, Monica [4 ]
Barros R, Claudio [2 ]
Moreno M, Ricardo [2 ]
机构：
[1] Univ Nacl Mayor San Marcos, Fac Ciencias Biol, Lab Biol Reprod Anim, Lima, Peru
[2] Pontificia Univ Catolica Chile, Fac Ciencias Biol, Dept Ciencias Fisiol, Santiago, Chile
[3] Univ Nacl Mayor San Marcos, Fac Med Vet, Lab Reprod, Lima, Peru
[4] Univ Chile, Fac Ciencias Vet & Pecuarias, Lab Reprod, Santiago, Chile
来源：
REVISTA DE INVESTIGACIONES VETERINARIAS DEL PERU | 2005年 / 16卷 / 02期
关键词：
dog sperm; acrosome reaction; membrane integrity; mitochondrial activity; chilled sperm;
D O I：
暂无
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
Preservation methods of spermatozoa using refrigeration have been developed for canine sperm; however, the rate of fertilization is still low, possibly due to cell damage for unknown reasons. The objective of this study was to evaluate the effect of refrigeration upon motility, mitochondrial activity, acrosomal membrane integrity, and acrosomal reaction in dog sperm incubated in three culture media (Ferp Talp, CCMm, Sp Talp) using new methodologies (Mito Tracker, Lyso Tracker, SBTI). The results indicated that exist a significant decrease (p<0.05) in motility, mitochondrial activity, and acrosomal membrane integrity in refrigerated sperm in comparison with fresh sperm. Also, the dynamic of acrosomal reaction was different in refrigerated sperm in comparison with fresh sperm. The Sp Talp and CCMm media showed the best results in holding the mitochondrial activity and acrosomal membrane integrity.
引用
收藏
页码：114 / 128
页数：15
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