HOMOLOGOUS NUCLEAR GENES ENCODE CYTOPLASMIC AND MITOCHONDRIAL GLUTAMINE-SYNTHETASE IN DROSOPHILA-MELANOGASTER

被引：51
作者：
CAIZZI, R [1 ]
BOZZETTI, MP [1 ]
CAGGESE, C [1 ]
RITOSSA, F [1 ]
机构：
[1] UNIV BOLOGNA,DEPARTIMENTO BIOL,I-40126 BOLOGNA,ITALY
来源：
JOURNAL OF MOLECULAR BIOLOGY | 1990年 / 212卷 / 01期
关键词：
D O I：
10.1016/0022-2836(90)90301-2
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We describe the cloning of the glutamine synthetase 1 (GS1) gene based on cross-homology with the glutamine synthetase 2 (GS2) gene in Drosophila melanogaster. We have determined the GS gene number in the Drosophila genome, and we describe the isolation of cDNA clones corresponding to the two isoforms, their entire sequence and their transcription pattern. We looked for subcellular localization of one enzymic isoform; in this way, we were able to locate the GS1 enzyme within the mitochondria of D. melanogaster. We have compared different GS sequences from plants and humans; emerging evolutionary implications are discussed. In addition, we have identified a certain highly stable secondary structure at the nucleotide level in the coding region of isoforms located in the organella. © 1990.
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页码：17 / 26
页数：10
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