Detection of Leishmania in the blood of early kala-azar patients with the aid of the polymerase chain reaction

被引:56
作者
Adhya, S [1 ]
Chatterjee, M [1 ]
Hassan, MQ [1 ]
Mukherjee, S [1 ]
Sen, S [1 ]
机构
[1] SEN MED RES CTR,PATNA 800001,BIHAR,INDIA
关键词
visceral leishmaniasis; Leishmania donovani; diagnosis; ELISA; PCR; microscopy; India;
D O I
10.1016/0035-9203(95)90416-6
中图分类号
R1 [预防医学、卫生学];
学科分类号
1004 ; 120402 ;
摘要
Samples from 39 patients with symptoms suggestive of early visceral leishmaniasis were independently assayed by microscopy of tissue smears, enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) of blood deoxyribonucleic acid. Of these patients, 19 were confirmed as positive or negative by all 3 tests; 11 patients (28%) negative by smear were positive by ELISA and PCR; and 7 (18%) were positive by PCR alone. These results demonstrate the high sensitivity of the nbn-invasive PCR and, to a smaller extent, ELISA, in the early diagnosis of visceral leishmaniasis.
引用
收藏
页码:622 / 624
页数:3
相关论文
共 14 条
[1]   DIRECT AGGLUTINATION-TEST FOR LEISHMANIASIS [J].
ALLAIN, DS ;
KAGAN, IG .
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1975, 24 (02) :232-236
[2]   DIAGNOSIS OF NEW-WORLD LEISHMANIASIS - SPECIFIC DETECTION OF SPECIES OF THE LEISHMANIA-BRAZILIENSIS COMPLEX BY AMPLIFICATION OF KINETOPLAST DNA [J].
DEBRUIJN, MHL ;
BARKER, DC .
ACTA TROPICA, 1992, 52 (01) :45-58
[3]  
DEBRUJIN MHL, 1993, TROP MED PARASITOL, V44, P201
[4]  
HASSAN MQ, 1993, PARASITOLOGY, V107, P509, DOI 10.1017/S0031182000068086
[5]   MICRO-ELISA TECHNIQUE IN SERODIAGNOSIS OF VISCERAL LEISHMANIASIS [J].
HOMMEL, M ;
PETERS, W ;
RANQUE, J ;
QUILICI, M ;
LANOTTE, G .
ANNALS OF TROPICAL MEDICINE AND PARASITOLOGY, 1978, 72 (03) :213-218
[6]   SERODIAGNOSTIC ASSAY FOR VISCERAL LEISHMANIASIS EMPLOYING MONOCLONAL-ANTIBODIES [J].
JAFFE, CL ;
MCMAHONPRATT, D .
TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 1987, 81 (04) :587-594
[7]  
Manson-Bahr P. E. C., 1987, The leishmaniases in biology and medicine. Volume II. Clinical aspects and control, P703
[8]   EVALUATION OF DIRECT AGGLUTINATION-TEST (DAT) AND ELISA FOR SERODIAGNOSIS OF VISCERAL LEISHMANIASIS IN INDIA [J].
PAL, A ;
MUKERJI, K ;
BASU, D ;
NASKAR, K ;
MALLICK, KK ;
GHOSH, DK .
JOURNAL OF CLINICAL LABORATORY ANALYSIS, 1991, 5 (05) :303-306
[9]   COMPARISON OF PCR WITH DIRECT EXAMINATION OF BONE-MARROW ASPIRATION, MYELOCULTURE, AND SEROLOGY FOR DIAGNOSIS OF VISCERAL LEISHMANIASIS IN IMMUNOCOMPROMISED PATIENTS [J].
PIARROUX, R ;
GAMBARELLI, F ;
DUMON, H ;
FONTES, M ;
DUNAN, S ;
MARY, C ;
TOGA, B ;
QUILICI, M .
JOURNAL OF CLINICAL MICROBIOLOGY, 1994, 32 (03) :746-749
[10]   AMPLIFICATION OF KINETOPLAST DNA AS A TOOL IN THE DETECTION AND DIAGNOSIS OF LEISHMANIA [J].
RODGERS, MR ;
POPPER, SJ ;
WIRTH, DF .
EXPERIMENTAL PARASITOLOGY, 1990, 71 (03) :267-275