THE BETA-TUBULIN GENES OF DROSOPHILA-AURARIA ARE ARRANGED IN A CLUSTER

被引：15
作者：
SCOURAS, ZG
MILIONI, D
YIANGOU, M
DUCHENE, M
DOMDEY, H
机构：
[1] UNIV MUNICH,GENZENTRUM,MOLEK BIOL LAB,D-82152 MARTINSRIED,GERMANY
[2] ARISTOTELIAN UNIV THESSALONIKI,FAC SCI,SCH BIOL,DEPT GENET DEV & MOLEC BIOL,GR-54006 THESSALONIKI,GREECE
来源：
CURRENT GENETICS | 1994年 / 25卷 / 01期
关键词：
BETA-TUBULIN GENES; GENE-CLUSTER; DROSOPHILA AURARIA;
D O I：
10.1007/BF00712973
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
When the beta 1-, beta 2- and beta 3-tubulin-specific DNAs from Drosophila melanogaster were used as probes to recognize tubulin-specific sequences in the chromosomes of Drosophila auraria, they were found to hybridize to the same polytene band in region 32C of the 2L polytene chromosome. Three overlapping clones were isolated from a lambda EMBL3 genomic library of D. auraria, and they all contain beta-tubulin-specific sequences based on hybridization and partial-sequencing experiments of subcloned fragments. These clones hybridize in situ to the same polytene chromosome band in region 32C and they represent an approximately 35-kb fragment of genomic DNA.
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页码：84 / 87
页数：4
相关论文
共 18 条
[1]   SCREENING GAMMAGT RECOMBINANT CLONES BY HYBRIDIZATION TO SINGLE PLAQUES INSITU [J].
BENTON, WD ;
DAVIS, RW .
SCIENCE, 1977, 196 (4286) :180-182
[2]  
BIOLOJAN S, 1984, EMBO J, V3, P2543
[3]  
CLEVELAND DW, 1985, ANNU REV BIOCHEM, V54, P331, DOI 10.1146/annurev.bi.54.070185.001555
[4]   A TECHNIQUE FOR RADIOLABELING DNA RESTRICTION ENDONUCLEASE FRAGMENTS TO HIGH SPECIFIC ACTIVITY [J].
FEINBERG, AP ;
VOGELSTEIN, B .
ANALYTICAL BIOCHEMISTRY, 1983, 132 (01) :6-13
[5]   INTRON AND UPSTREAM SEQUENCES REGULATE EXPRESSION OF THE DROSOPHILA-BETA-3-TUBULIN GENE IN THE VISCERAL AND SOMATIC MUSCULATURE, RESPECTIVELY [J].
GASCH, A ;
HINZ, U ;
RENKAWITZPOHL, R .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (09) :3215-3218
[6]   THE EXPRESSION OF BETA-1 AND BETA-3 TUBULIN GENES OF DROSOPHILA-MELANOGASTER IS SPATIALLY REGULATED DURING EMBRYOGENESIS [J].
GASCH, A ;
HINZ, U ;
LEISS, D ;
RENKAWITZPOHL, R .
MOLECULAR & GENERAL GENETICS, 1988, 211 (01) :8-16
[7]   DUPLICATIONS IN THE POLYTENE CHROMOSOMES OF DROSOPHILA-AURARIA [J].
KASTRITSIS, CD ;
SCOURAS, ZG ;
ASHBURNER, M .
CHROMOSOMA, 1986, 93 (04) :381-385
[8]  
LEISS D, 1988, DEVELOPMENT, V104, P525
[9]  
LEUMEUNIER F, 1986, GENET BIOL DROSOPHIL, V3, P148
[10]  
MANIATIS T, 1982, MOL CLONING
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