LIVER TRIGLYCERIDE CONCENTRATION AND BODY PROTEIN-METABOLISM IN ETHANOL-TREATED RATS - EFFECT OF ENERGY AND NUTRIENT SUPPLEMENTATION

The objective of this study was to compare the metabolic effects of long-term ethanol consumption with oral (Lieber-DeCarli) and enteral feeding techniques. Enteral feeding allowed administration of greater amounts of energy and nutrients. After 21 days of treatment using the Lieber-DeCarli technique, the ethanol-treated rats had the following significant (P < 0.05) differences from pair-fed controls: lower cumulative nitrogen balance (days 5-21; 2.8 ± 0.1 g N vs. 3.5 ± 0.1 g N), lower protein content of gastrocnemius muscle (289 ± 17 mg vs. 358 ± 11 mg) and intestinal mucosa (461 ± 19 mg vs. 577 ± 40 mg), higher plasma leucine concentration (147 ± 8 μmol/L vs. 102 ± 8 μmol/L), higher liver protein content (2222 ± 122 mg vs. 1679 ± 58 mg), and higher liver triglyceride concentration (38.4 ± 2.8 mg/g vs. 8.7 ± 1.0 mg/g). When rats received the same amount of nitrogen (1.5 g · kg-1 · day-1) and ethanol (13 g · kg-1 · day-1) but 16.3% more energy and nutrients by a surgically implanted gastric cannula (enterally fed), the effects of ethanol on nitrogen balance, tissue protein content, plasma leucine concentration, and liver triglyceride concentration were similar to those observed in the rats fed orally. It is concluded that the metabolic effects observed using the Lieber-DeCarli feeding technique are due to ethanol per se and not the synergism of ethanol and undernutrition as recently suggested. © 1992.