THE CHLOROPLAST BETA-SUBUNIT ALLOWS ASSEMBLY OF THE ESCHERICHIA-COLI F0-PORTION OF THE ENERGY TRANSDUCING ADENOSINE-TRIPHOSPHATASE

被引：11
作者：
MUNN, AL
WHITFELD, PR
BOTTOMLEY, W
HUDSON, GS
JANS, DA
GIBSON, F
COX, GB
机构：
[1] AUSTRALIAN NATL UNIV,JOHN CURTIN SCH MED RES,DIV BIOCHEM & MOLEC BIOL,GPO BOX 334,CANBERRA,ACT 2601,AUSTRALIA
[2] CSIRO,DIV PLANT IND,CANBERRA,ACT 2601,AUSTRALIA
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1991年 / 1060卷 / 01期
关键词：
ATPASE; F0F1-; BETA-SUBUNIT; CHLOROPLAST; (ESCHERICHIA-COLI);
D O I：
10.1016/S0005-2728(05)80122-7
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The effect of the expression of the chloroplast F1-ATPase beta-subunit in two Escherichia coli beta-subunit mutant strains was investigated. The amount of chloroplast beta-subunit formed in E. coli was increased by introducing 'Shine-Dalgarno' sequence upstream from the translation start site. The chloroplast beta-subunit was membrane-bound but was unable to functionally replace the mutant beta-subunit in a strain carrying the uncD409 allele. However, in an E. coli mutant strain unable to form the beta- and epsilon-subunits the presence of the chloroplast beta-subunit enabled the assembly of a functional proton pore.
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页码：82 / 88
页数：7
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