SUBSTRATE-SPECIFICITY OF ALPHA-GALACTOSIDASE

被引：0

作者：

ZAPROMETOVA, OM ^{[1
]}

ULEZLO, IV ^{[1
]}

LIKHOSHERSTOV, LM ^{[1
]}

MARYNOVA, MD ^{[1
]}

机构：

[1] ND ZELINSKII ORGAN CHEM INST, MOSCOW, USSR

来源：

BIOCHEMISTRY-MOSCOW | 1990年 / 55卷 / 12期

关键词：

ALPHA-GALACTOSIDASE; SUBSTRATE SPECIFICITY; CEPHALOSPORIUM ACREMONIUM;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The ability of alpha-galactosidases of the micromycete Cephalosporium acremonium, green coffee beans, and sweet almond fruits to split off alpha-1,6-, alpha-1,4-, and alpha-1,3-bonded galactose residues of simple and complex natural substrates (melibiose, raffinose, trihexocide ceramide, Gal-alpha-1,3Gal,Gal-alpha-1,3(Fuc-alpha-1,2)Gal, group-specific glycoprotein B) was studied. All three enzymes investigated split off both alpha-1,3-, alpha-1,4-, and alpha-1,6-bonded galactose residues from substrates with a linear structure; however, only the alpha-galactosidase of green coffee beans acted on branched trisaccharide. The sensitivity of the alpha-galactosidases to the presence of branching of the carbon chain was demonstrated for the first time.

引用

页码：1702 / 1706

页数：5

共 19 条

[1] BETA-GALACTOSIDASES FROM JACK BEAN MEAL AND ALMOND EMULSIN - APPLICATION FOR ENZYMATIC DISTINCTION OF GALBETA1-]4G1CNAC AND GALBETA1-]3G1CNAC LINKAGES [J].