THE DETERMINATION OF THE MOLECULAR-WEIGHT DISTRIBUTION OF PECTINS BY CALIBRATED GPC .1. CALIBRATION BY LIGHT-SCATTERING AND MEMBRANE OSMOMETRY

被引：21

作者：

BERTH, G ^{[1
]}

DAUTZENBERG, H ^{[1
]}

LEXOW, D ^{[1
]}

ROTHER, G ^{[1
]}

机构：

[1] ACAD SCI GDR,INST POLYMER CHEM,TELTOW,GERMANY

来源：

CARBOHYDRATE POLYMERS | 1990年 / 12卷 / 01期

关键词：

D O I：

10.1016/0144-8617(90)90103-Y

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Commercial high methoxyl citrus pectin was fractionated on Sepharose 2 B/Sepharose 4 B. Fractions were used to calibrate GPC by light scattering and membrane osmometry. The angular dependence of light scattering of the fractions was interpreted in terms of a two-component system giving reliable Mw/Mn ratios for the major molecularly dispersed component which forms coils of considerable stiffness in solution. A minor particulate spherical component was identified which was rich in neutral sugars. Structural parameters of this component were determined by comparison of the experimental scattering behavior with master curves. β-Elimination of the fully esterified pectin was carried out to reveal the chemical nature of the particulate component. © 1989.

引用

页码：39 / 59

页数：21

共 41 条

[1] GEL-PERMEATION CHROMATOGRAPHY AND THE MARK-HOUWINK RELATION FOR PECTINS WITH DIFFERENT DEGREES OF ESTERIFICATION [J].