CHARACTERIZATION OF ENDOGENOUS SODIUM-CHANNEL GENE EXPRESSED IN CHINESE-HAMSTER OVARY CELLS

被引：25

作者：

LALIK, PH ^{[1
]}

KRAFTE, DS ^{[1
]}

VOLBERG, WA ^{[1
]}

CICCARELLI, RB ^{[1
]}

机构：

[1] STERLING WINTHROP RES INST,DIV RES,DEPT CARDIOVASC PHARMACOL,RENSSELAER,NY 12144

来源：

AMERICAN JOURNAL OF PHYSIOLOGY | 1993年 / 264卷 / 04期

关键词：

ION CHANNEL; CHO-1; ELECTROPHYSIOLOGY; TETRODOTOXIN;

D O I：

10.1152/ajpcell.1993.264.4.C803

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

Chinese hamster ovary (CHO-K1) cells were observed to display transient inward Na+ currents of average amplitude (-92 +/- 20 pA), which activated at voltages more than -40 mV, and peak inward currents were observed at potentials equal to or more than +10 mV. Inward Na+ currents in these cells were eliminated after treatment with 500 or 50 nM tetrodotoxin (TTX), whereas 5 nM TTX resulted in 64 +/- 10% inhibition of Na+ current. Using DNA primers designed to bind to the rat brain IIA Na+ channel subtype, we amplified specific polymerase chain reaction (PCR) fragments from CHO-K1 poly-(A)+RNA. The cloning and sequencing of two of these fragments confirmed the presence of an endogenously expressed Na+ channel gene in these cells, which we have termed cho 1. Comparison of the DNA sequence of cho 1 PCR fragments with other known Na+ channel genes indicated a high degree of homology with rat brain Na+ channel subtypes. Northern blots using riboprobes generated from the cho 1 PCR fragments revealed the presence of a specific 9-kb mRNA in these cells. The molecular and electrophysiological data suggest that the cho 1 Na+ channel gene from CHO-K1 cells is closely related to brain-type Na+ channels.

引用

页码：C803 / C809

页数：7

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