TRANSOVARIAN TRANSMISSION OF A FOREIGN GENE IN THE SILKWORM, BOMBYX-MORI, BY AUTOGRAPHA-CALIFORNICA NUCLEAR POLYHEDROSIS-VIRUS

被引：19

作者：

MORI, H ^{[1
]}

YAMAO, M ^{[1
]}

NAKAZAWA, H ^{[1
]}

SUGAHARA, Y ^{[1
]}

SHIRAI, N ^{[1
]}

MATSUBARA, F ^{[1
]}

SUMIDA, M ^{[1
]}

IMAMURA, T ^{[1
]}

机构：

[1] NIKKO MKT CO LTD,KYOTO 601,JAPAN

来源：

BIO-TECHNOLOGY | 1995年 / 13卷 / 09期

关键词：

D O I：

10.1038/nbt0995-1005

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We introduced a firefly luciferase gene, expressed under control of Drosophila heat shock protein gene promoter, into Autographa californica nuclear polyhedrosis virus (AcNPV). When the 5th instar larvae of the silkworm, Bombyx mori, were inoculated with the recombinant virus, luciferase activities were detected in the virus-infected larvae and pupae, and in the newly hatched larvae of the next generation. PCR amplification and Southern blot hybridization analysis demonstrated that the luciferase gene was transmitted through at least the F-2 generation. In addition, the V-cathepsin gene, encoding a cysteine protease of AcNPV, was also detected in the DNA of all individuals of the F-2 generation. These results show that AcNPV can be utilized as vector for the transovarian transmission of foreign genes in the silkworm.

引用

页码：1005 / 1007

页数：3

共 21 条

[1] THE COMPLETE DNA-SEQUENCE OF AUTOGRAPHA-CALIFORNICA NUCLEAR POLYHEDROSIS-VIRUS [J].