ESCHERICHIA-COLI KGTP ENCODES AN ALPHA-KETOGLUTARATE TRANSPORTER

被引：62
|
作者：
SEOL, W
SHATKIN, AJ
机构：
[1] Ctr. for Adv. Biotech. and Medicine, Piscataway, NJ 08854-5638
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 09期
关键词：
WITA; MEMBRANE PROTEIN; COMPLEMENTATION; CITA;
D O I：
10.1073/pnas.88.9.3802
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The witA gene located between pss and rrnG on the Escherichia coli chromosome encodes a 432-amino acid protein. It is homologous to a human hepatoma glucose transporter and to E. coli membrane proteins that transport citrate (CitA), arabinose (AraE), and xylose (XylE), and, like these carrier proteins, WitA also contains 12 highly hydrophobic putative membrane-spanning regions. Gene disruption mutants constructed in two E. coli strains grew slowly or not at all, depending on genetic background, in M9 minimal medium containing alpha-ketoglutarate. Growth on alpha-ketoglutarate and uptake of alpha-[C-14]ketoglutarate were restored by transformation with plasmids containing witA. These complementation studies indicate that WitA is an alpha-ketoglutarate transporter and should be renamed kgtP(alpha-ketoglutarate permease).
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页码：3802 / 3806
页数：5
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