COPPER-ZINC SUPEROXIDE-DISMUTASE FROM ASCARIS-SUUM (NEMATODA) - PURIFICATION AND CHARACTERIZATION

被引：1
|
作者：
SANCHEZMORENO, M [1 ]
ENTRALA, E [1 ]
JANSSEN, D [1 ]
OSUNA, A [1 ]
机构：
[1] UNIV GRANADA,FAC SCI,MOLEC PARASITOL LAB,E-18071 GRANADA,SPAIN
来源：
BIOSCIENCE REPORTS | 1994年 / 14卷 / 02期
关键词：
COPPER-ZINC SUPEROXIDE DISMUTASE; ASCARIS SUUM; PURIFICATION AND CHARACTERIZATION;
D O I：
10.1007/BF01210303
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Copper-zinc superoxide dismutase from Ascaris suum (Nematoda) was purified in a new, more efficient. and Easter manner. The process included differential centrifugation, fractionation with ammonium sulfate, and sodium dodecyl sulfate-polyacrylamide electrophoresis, yielding a 340-fold purification (specific activity of 47 units/mg). Optimal storage conditions, optimal pH range, thermostability, molecular weight and ultravioltet-visible absorption spectrum of the enzyme are described, and a new enzymatic model for pharmacological screening is suggested.
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收藏
页码：83 / 90
页数：8
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