A DNA-PROBE FOR IDENTIFICATION OF PYTHIUM IRREGULARE IN SOIL

被引：15
|
作者：
MATTHEW, J [1 ]
HAWKE, BG [1 ]
PANKHURST, CE [1 ]
机构：
[1] CSIRO,COOPERAT RES CTR SOIL & LAND MANAGEMENT,GLEN OSMOND,SA 5064,AUSTRALIA
来源：
MYCOLOGICAL RESEARCH | 1995年 / 99卷
关键词：
D O I：
10.1016/S0953-7562(09)80717-X
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A DNA probe recognizing a repeated sequence was isolated from a genomic library of the oomycete Pythium irregulare. This probe was tested for reactivity with DNA from a variety of Pythium spp. and was shown to react only with P. irregulare and P. spinosum. Isolates of P. irregulare showed variation in their restriction patterns when tested with this probe. Variation in restriction patterns was also noted for isolates of P. irregulare obtained from soil under wheat pasture and woodland.
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页码：579 / 584
页数：6
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