HIGH-LEVEL SOLUBLE EXPRESSION OF ISOPENICILLIN-N SYNTHASE ISOZYMES IN ESCHERICHIA-COLI

被引：21

作者：

BALDWIN, JE ^{[1
]}

BLACKBURN, JM ^{[1
]}

SUTHERLAND, JD ^{[1
]}

WRIGHT, MC ^{[1
]}

机构：

[1] OXFORD CTR MOLEC SCI,OXFORD OX1 3QY,ENGLAND

来源：

TETRAHEDRON | 1991年 / 47卷 / 31期

关键词：

D O I：

10.1016/S0040-4020(01)86491-7

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

The Isopenicillin N Synthase genes from Cephalosporium acremonium, Penicillium chrysogenum and Aspergillus nidulans have been expressed at high levels in Escherichia coli under control of the trc or lac promoters. The enzymes are produced in soluble form in contrast to previously reported expression in insoluble form under control of the lambda-P(L) promoter.

引用

收藏

页码：5991 / 6002

页数：12

相关论文

共 30 条

[1] NUCLEOTIDE-SEQUENCE ANALYSIS OF THE CHLORAMPHENICOL RESISTANCE TRANSPOSON TN9 [J].

ALTON, NK ;

VAPNEK, D .

NATURE, 1979, 282 (5741) :864-869

[2]

BALDWIN JE, 1990, BIOCHEM J, V266, P561

[3] PENICILLIN BIOSYNTHESIS - DUAL PATHWAYS FROM A MODIFIED SUBSTRATE [J].

BALDWIN, JE ;

ABRAHAM, EP ;

ADLINGTON, RM ;

CHAKRAVARTI, B ;

DEROME, AE ;

MURPHY, JA ;

FIELD, LD ;

GREEN, NB ;

TING, HH ;

USHER, JJ .

JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS, 1983, (22) :1317-1319

[4] THE BIOSYNTHESIS OF PENICILLINS AND CEPHALOSPORINS [J].

BALDWIN, JE ;

ABRAHAM, E .

NATURAL PRODUCT REPORTS, 1988, 5 (02) :129-145

[5] EVIDENCE FOR EPOXIDE FORMATION FROM ISOPENICILLIN-N SYNTHASE [J].

BALDWIN, JE ;

ADLINGTON, RM ;

BRADLEY, M ;

TURNER, NJ ;

PITT, AR .

JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS, 1989, (15) :978-981

[6]

BALDWIN JE, 1990, FEMS MICROBIOL LETT, V68, P45

[7] PURIFICATION AND CHARACTERIZATION OF CLONED ISOPENICILLIN-N SYNTHETASE [J].

BALDWIN, JE ;

KILLIN, SJ ;

PRATT, AJ ;

SUTHERLAND, JD ;

TURNER, NJ ;

CRABBE, MJC ;

ABRAHAM, EP ;

WILLIS, AC .

JOURNAL OF ANTIBIOTICS, 1987, 40 (05) :652-659

[8]

BALDWIN JE, 1989, PHARM FPLC NEWS

[9] CLONING, SEQUENCE-ANALYSIS AND TRANSCRIPTIONAL STUDY OF THE ISOPENICILLIN-N SYNTHASE OF PENICILLIUM-CHRYSOGENUM AS-P-78 [J].

BARREDO, JL ;

CANTORAL, JM ;

ALVAREZ, E ;

DIEZ, B ;

MARTIN, JF .

MOLECULAR & GENERAL GENETICS, 1989, 216 (01) :91-98

[10]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3