TRANSFORMATION OF FRESH-WATER AND MARINE CAULOBACTERS BY ELECTROPORATION

被引：61

作者：

GILCHRIST, A ^{[1
]}

SMIT, J ^{[1
]}

机构：

[1] UNIV BRITISH COLUMBIA,DEPT MICROBIOL,VANCOUVER V6T 1W5,BC,CANADA

来源：

JOURNAL OF BACTERIOLOGY | 1991年 / 173卷 / 02期

关键词：

D O I：

10.1128/jb.173.2.921-925.1991

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We performed plasmid electrotransformation of Caulobacter crescentus strains and obtained up to 3 x 10(8) transformants per mu-g of pKT230. The presence and integrity of the paracrystalline protein surface (S) layer influenced electroporation; caulobacters lacking the S layer were electrotransformed 10 times more efficiently than caulobacters possessing the S layers. A procedure yielding 1,500 transformants per mu-g of pKT230 was developed for a marine caulobacter. Electroporation was used in combination with several genetic techniques, including introduction of ligation mixtures, suicide transposon mutagenesis, gene replacement, and plasmid electrotransfer from Escherichia coli to caulobacters.

引用

页码：921 / 925

页数：5

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