CHARACTERIZATION AND IMMUNOGENICITY OF EX880, A SALMONELLA-TYPHI TY21A-BASED CLONE WHICH PRODUCES VIBRIO-CHOLERAE O-ANTIGEN

被引：14

作者：

ATTRIDGE, SR ^{[1
]}

DEARLOVE, C ^{[1
]}

BEYER, L ^{[1
]}

VANDENBOSCH, L ^{[1
]}

HOWLES, A ^{[1
]}

HACKETT, J ^{[1
]}

MORONA, R ^{[1
]}

LABROOY, J ^{[1
]}

ROWLEY, D ^{[1
]}

机构：

[1] UNIV ADELAIDE,DEPT MED,ADELAIDE,SA 5001,AUSTRALIA

来源：

INFECTION AND IMMUNITY | 1991年 / 59卷 / 07期

关键词：

D O I：

10.1128/IAI.59.7.2279-2284.1991

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

EX645 is a derivative of Salmonella typhi Ty21a which carries a plasmid specifying production of Vibrio cholerae O antigen. When cultured with exogenous galactose to overcome the galE defect of the vector, EX645 also synthesizes S. typhi O antigen, and this can result in the masking of the shorter V. cholerae O antigen on the bacterial surface. To determine whether the potential for such masking at least partly underlies the inconsistency of anti-V. cholerae responses elicited by EX645, a derivative of this strain has been isolated, characterized, and tested for immunogenicity in human volunteers. EX880 has an rfb defect which prevents synthesis of S. typhi O antigen, and consequently V. cholerae O antigen is still detectable on the surface of the clone following growth in the presence of galactose. Compared with EX645, EX880 more consistently elicited significant rises in serum bactericidal antibody levels, although individual responses within a cohort still varied widely.

引用

页码：2279 / 2284

页数：6

共 22 条

[1] SURFACE COEXPRESSION OF VIBRIO-CHOLERAE AND SALMONELLA-TYPHI O-ANTIGENS ON TY21A CLONE EX210 [J].