ELECTROPHORETIC KARYOTYPING WITHOUT THE NEED FOR GENERATING PROTOPLASTS

被引：43
作者：
MCCLUSKEY, K
RUSSELL, BW
MILLS, D
机构：
[1] OREGON STATE UNIV,DEPT BOT & PLANT PATHOL,CORVALLIS,OR 97331
[2] OREGON STATE UNIV,GENET PROGRAM,CORVALLIS,OR 97331
来源：
CURRENT GENETICS | 1990年 / 18卷 / 04期
关键词：
Intact cells; Molecular karyotype; PFGE; Phytopathogenic fungi;
D O I：
10.1007/BF00318221
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Chromosome samples for pulsed-field electrophoresis have been prepared without first generating protoplasts. The technique involves treatment of intact, agarose-solidified cell material with protease in the presence of EDTA and SDS. Saccharomyces cerevisiae, Ustilago hordei, Tilletia caries, and T. controversa karyotypes are clearly resolved with this technique. Colonies of U. hordei and S. cerevisiae removed from the surface of agarsolidified media and prepared for PFGE by this abbreviated method also yield well resolved karyotypes. © 1990 Springer-Verlag.
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页码：385 / 386
页数：2
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