NEW DISTAL MARKER CLOSELY LINKED TO THE FRAGILE-X LOCUS

被引：14

作者：

HULSEBOS, TJM

OOSTRA, BA

BROERSEN, S

SMITS, A

VANOOST, BA

WESTERVELD, A

机构：

[1] ERASMUS UNIV,DEPT CELL BIOL,3000 DR ROTTERDAM,NETHERLANDS

[2] CATHOLIC UNIV NIJMEGEN,RADBOUD HOSP,DEPT HUMAN GENET,NIJMEGEN,NETHERLANDS

来源：

HUMAN GENETICS | 1991年 / 87卷 / 03期

关键词：

D O I：

10.1007/BF00200922

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have isolated II-10, a new X-chromosomal probe that identifies a highly informative two-allele TaqI restriction fragment length polymorphism at locus DXS466. Using somatic cell hybrids containing distinct portions of the long arm of the X chromosome, we could localize DXS466 between DXS296 and DXS304, both of which are closely linked distal markers for fragile X. This regional localization was supported by the analysis, in fragile X families, of recombination events between these three loci, the fragile X locus and locus DXS52, the latter being located at a more distal position. DXS466 is closely linked to the fragile X locus with a peak lod score of 7.79 at a recombination fraction of 0.02. Heterozygosity of DXS466 is approximately 50%. Its close proximity and relatively high informativity make DXS466 a valuable new diagnostic DNA marker for fragile X.

引用

页码：369 / 372

页数：4

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