DIFFICULTIES ENCOUNTERED IN THE DETECTION OF NITRIC-OXIDE (NO) BY SPIN TRAPPING TECHNIQUES - A CAUTIONARY NOTE

被引：63
作者：
ARROYO, CM
KOHNO, M
机构：
[1] VET ADM MED CTR,BALTIMORE,MD 21218
[2] JEOL LTD,DIV ANALYT INSTRUMENTS,ESR APPLICAT LAB,TOKYO 196,JAPAN
来源：
FREE RADICAL RESEARCH COMMUNICATIONS | 1991年 / 14卷 / 02期
关键词：
SPIN TRAPPING; NITRIC OXIDE; ENDOTHELIUM-DERIVED RELAXING FACTOR (EDRF); NITRONE NITROSO SPIN TRAPS; ESR;
D O I：
10.3109/10715769109094127
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The spin trapping technique was used in an attempt to detect the free radical nitric oxide (NO) in solution. Five different spin traps were examined, αphenyl-N-tert butyl nitrone (PBN), α4-pyridyl-N-oxide) N-tert-butylnitrone (POBN), 5,5-dimethyl-pyrroline-N-oxide (DMPO), 2-methyl-2-nitrosopropane (MNP) and 3,5-dibromo-4-nitrosobenzene sulfonate (DBNBS). Our results suggest that the nitroso spin traps (MNP, DBNBS) are better suited for the identification of NO-related signals, than the nitrones, DMPO, PBN and POBN. In addition, it is shown that spin trapping of NO-related signals with nitroso and nitrone spin traps is subject to many artifacts. © 1991 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.
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收藏
页码：145 / 155
页数：11
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