MOLECULAR-CLONING AND PHYSICAL MAPPING OF THE GENOME OF BOMBYX-MORI NUCLEAR POLYHEDROSIS-VIRUS

被引：71
作者：
MAEDA, S
MAJIMA, K
机构：
来源：
JOURNAL OF GENERAL VIROLOGY | 1990年 / 71卷
关键词：
D O I：
10.1099/0022-1317-71-8-1851
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A restriction fragment library which covered the entire genome of Bombyx mori nuclear polyhedrosis virus (BmNPV) was constructed using plasmid vectors. By analysis of cloned and viral DNA by double digestion with endonucleases and by hybridization techniques, a complete physical map of BmNPV was constructed for BamHI, EcoRI, HindIII, KpnI, PstI and SmaI. Five regions of repeated sequences containing EcoRI sites were also found and mapped.
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页码：1851 / 1855
页数：5
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