HMGCOA REDUCTASE AND CHOLESTEROL 7-ALPHA-HYDROXYLASE IN HUMAN-LIVER

被引:8
作者
BOSISIO, E
CIGHETTI, G
KIENLE, MG
TRITAPEPE, R
GALLI, G
机构
[1] UNIV MILAN, FAC PHARMACOL & PHARMACOGNOSY, I-20129 MILAN, ITALY
[2] UNIV MILAN, DEPT MED CHEM & BIOCHEM, I-20133 MILAN, ITALY
[3] UNIV MILAN, SCH MED, SURG CLIN 3, I-20122 MILAN, ITALY
关键词
D O I
10.1016/0024-3205(84)90372-2
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
The activities of HMGCoA [3-hydroxy-3-methylglutarylCoA] reductase and cholesterol 7.alpha.-hydroxylase were assayed in liver biopsies of patients with or without cholestyramine treatment. The active dephosphorylated form of HMGCoA reductase and the activity of cholesterol 7.alpha.-hydroxylase were under the detection limits in untreated subjects. After cholestyramine treatment activities of both enzymes were stimulated and the active form of HMGCoA reductase became detectable in 4 out of the 5 tested patients. In 2 subjects who received cholestyramine, the effect of exogenously added [4-14C]cholesterol on cholesterol 7.alpha.-hydroxylase was tested. In the presence of Tween 80, the detergent by which [14C]cholesterol was suspended, the enzyme activity was profoundly inhibited and synthesis of 7.alpha.-hydroxycholesterol was extremely low.
引用
收藏
页码:2075 / 2081
页数:7
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