EFFECT OF PHYTOREGULATORS AND PHYSIOLOGICAL-CHARACTERISTICS OF THE EXPLANTS ON MICROPROPAGATION OF MAYTENUS-ILICIFOLIA

被引:19
作者
PEREIRA, AMS [1 ]
MORO, JR [1 ]
CERDEIRA, RMM [1 ]
FRANCA, SC [1 ]
机构
[1] UNIV NACL ESTADUAL SAO PAULO,DEPT APPL BIOL,JABOTICABAL,SP,BRAZIL
关键词
ESPINHEIRA SANTA; MICROPROPAGATION; TISSUE CULTURE;
D O I
10.1007/BF00030003
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Micropropagated shoots of Maytenus ilicifolia Mart. were obtained from axillary buds cultured in Murashige & Skoog medium supplemented with 13.3 mu M 6-benzyladenine (BA). Addition of 1.1 mu M 1-indole-3-acetic acid (IAA) to the medium increased shoot elongation. The number of shoots formed was influenced by BA concentration, degree of juvenility of the explant, and by bud explant position on the stem. Cultures of buds taken from stem parts located close to the shoot tip yielded more callus than shoots, whereas axillary buds at distant positions from the apical bud yielded more shoots.
引用
收藏
页码:295 / 297
页数:3
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