LOCALIZATION OF PLANT LIPIDS FOR LIGHT-MICROSCOPY USING PARA-PHENYLENEDIAMINE IN TISSUES OF ARACHIS-HYPOGAEA L

被引：17
作者：
BAL, AK
机构：
[1] Department of Biology, Memorial University of Newfoundland, St. John's
来源：
STAIN TECHNOLOGY | 1990年 / 65卷 / 02期
关键词：
D O I：
10.3109/10520299009108062
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
p-Phenylenediamine (pPD) can be used en bloc to preserve and differentiate cell lipids in aldehyde-fixed peanut plant tissues treated with osmium tetroxide during dehydration in 70% ethanol. Semithin plastic sections for light microscopy need o further staining and can be mounted in Histoclad after drying on a slide. Brown staining above background differentiates lipid-containing structures. Nonspecific staining can be distinguished in control preparations extracted en bloc with lipid solvents. © 1990 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.
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页码：91 / 94
页数：4
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