LOCALIZATION OF PLANT LIPIDS FOR LIGHT-MICROSCOPY USING PARA-PHENYLENEDIAMINE IN TISSUES OF ARACHIS-HYPOGAEA L

被引：17

作者：

BAL, AK

机构：

[1] Department of Biology, Memorial University of Newfoundland, St. John's

来源：

STAIN TECHNOLOGY | 1990年 / 65卷 / 02期

关键词：

D O I：

10.3109/10520299009108062

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

p-Phenylenediamine (pPD) can be used en bloc to preserve and differentiate cell lipids in aldehyde-fixed peanut plant tissues treated with osmium tetroxide during dehydration in 70% ethanol. Semithin plastic sections for light microscopy need o further staining and can be mounted in Histoclad after drying on a slide. Brown staining above background differentiates lipid-containing structures. Nonspecific staining can be distinguished in control preparations extracted en bloc with lipid solvents. © 1990 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

引用

页码：91 / 94

页数：4

共 9 条

[1] ULTRASTRUCTURAL CHARACTERISTICS OF THE HOST-SYMBIONT INTERFACE IN NITROGEN-FIXING PEANUT NODULES [J].