USE OF NINHYDRIN AS A REAGENT FOR REVERSIBLE MODIFICATION OF ARGININE RESIDUES IN PROTEINS

被引：36

作者：

CHAPLIN, MF ^{[1
]}

机构：

[1] UNIV GHANA, DEPT BIOCHEM, LEGON, GHANA

来源：

BIOCHEMICAL JOURNAL | 1976年 / 155卷 / 02期

关键词：

D O I：

10.1042/bj1550457

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple technique was developed for the specific reversible modification of guanidino groups in proteins involving reaction with ninhydrin. The extent of the reaction is easily determined non-destructively by spectrophotometric analysis. The reagent can be used for the titration of sterically unhindered thiol groups in proteins.

引用

页码：457 / 459

页数：3

共 7 条

[1]

ARNON R., 1970, METHODS ENZYMOL, V19, P226, DOI [DOI 10.1016/0076-6879(70)19017-3, 10.1016/0076-6879(70)19017-3]

[2]

HEINRIKSON RL, 1971, J BIOL CHEM, V246, P4090

[3]

HEINRIKSON RL, 1974, PROGR BIOORGANIC CHE, V3, P141

[4]

MACFADYEN DA, 1950, J BIOL CHEM, V186, P1

[5] Triketohydrindene hydrate [J].

Ruhemann, S .

JOURNAL OF THE CHEMICAL SOCIETY, 1910, 97 :2025-2031

[6]

Walsh K.A., 1970, Methods in Enzymology, V19, P41, DOI 10.1016/0076-6879(70)19006-9

[7]

Yankeelov J A Jr, 1972, Methods Enzymol, V25, P566, DOI 10.1016/S0076-6879(72)25056-X

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