HISTONE ACETYLATION AND GLOBIN GENE SWITCHING

被引:100
作者
HEBBES, TR [1 ]
THORNE, AW [1 ]
CLAYTON, AL [1 ]
CRANEROBINSON, C [1 ]
机构
[1] PORTSMOUTH POLYTECH,BIOPHYS LABS,ST MICHAELS BLDG,WHITE SWAN RD,PORTSMOUT P01 2DT,ENGLAND
关键词
D O I
10.1093/nar/20.5.1017
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
An affinity-purified antibody that recognises the epitope epsilon-acetyl lysine has been used to fractionate chicken erythrocyte mononucleosomes obtained from 5 and 15 day embryos. The antibody bound chromatin was enriched in multiply acetylated forms of the core histones H3, H4 and H2B, but not in ubiquitinated H2A. The DNA of these modified nucleosomes was probed with genomic sequences from the embryonic beta(e) gene (active at 5 days) and from the adult beta(A) gene (active at 15 days). Both genes were found to be highly enriched in the acetylated nucleosomes fractionated from both 5 day and from 15 day erythrocytes. We conclude that globin switching is not linked to a change in acetylation status of the genes and that a 'poised' gene carries histones acetylated to a similar level as a transcriptionally active gene. Core histone acetylation is not therefore a direct consequence of the transcriptional process and might operate at the level of the globin locus as a general enabling step for transcription.
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收藏
页码:1017 / 1022
页数:6
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