A HIGH-EFFICIENCY METHOD FOR PURIFICATION AND ASSAY OF BEE VENOM PHOSPHOLIPASE-A2

被引：10

作者：

AMERATUNGA, RV

HAWKINS, R

PRESTIDGE, R

MARBROOK, J

机构：

[1] The Department of Molecular Medicine, University of Auckland, Auckland

来源：

PATHOLOGY | 1995年 / 27卷 / 02期

关键词：

D O I：

10.1080/00313029500169782

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

Phospholipase A(2) (PLA(2)) is the main allergen of hymenopteran venoms. We describe a highly efficient reverse phase high performance liquid chromatographic method (HPLC) for isolating PLA(2) from crude bee venom. This method removes all detectable contaminants such as melittin from PLA(2) while preserving the hemolytic action of PLA(2). In addition we describe a simple functional assay of PLA(2) based on its propensity to cause hemolysis of guina pig red blood cells. These techniques are particularly well suited to the isolation and assessment of PLA(2) of venoms which are available in limited quantities.

引用

页码：157 / 160

页数：4

共 9 条

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[2]

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[3]

LANGTON S R, 1990, Australian Journal of Medical Laboratory Science, V11, P78

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